Journal: PeerJ

Article Title: CircHIPK3 regulates cardiac fibroblast proliferation, migration and phenotypic switching through the miR-152-3p/TGF-β2 axis under hypoxia

doi: 10.7717/peerj.9796

Figure Lengend Snippet: To determine the role of miR-152-3p in CFs, CFs were transfected with the miR-152-3p mimic or inhibitor. (A–G and J–P) Representative images of EdU-stained CFs from different groups. Quantification of EdU+ cells presented as the % EdU-positive cells and Hoechst-stained nuclei. The orange color is EdU-positive cells, and the blue color is nuclei stained by Hoechst 33342. Scale bar = 20 μm. n = 10. * P < 0.05, vs. MNC group or INC group. (H, I and Q, R) The protein expression levels of Col I and Col III were measured by western blot analysis. n = 3. * P < 0.05, vs. MNC group or INC group. (S) Relative expression of miR-152-3p in CFs after transfection with miR-152-3p mimic or inhibitor was examined by RT-qPCR analysis. n = 3. * P < 0.05, vs. hypoxia group. (T) TGF-β2 was predicted as a target gene of miR-152-3p using the TargetScan database. HEK-293T cells were cotransfected with RLuc-TGF-β2-WT or RLuc-TGF-β2-Mut and miR-152-3p mimics or NC mimic. Luciferase activity was detected using the dual luciferase reporter assay at 48 h post-transfection. n = 3. * P < 0.05, vs. TGF-β2-WT + NC group. # P < 0.05, vs. TGF-β2-WT + miR-152-3p group. (U–X) The protein expression level of TGF-β2 was measured by western blot analysis. n = 3. * P < 0.05, vs. MNC group or INC group.

Article Snippet: The primary anti-bodies: Anti-Collagen I (Cell Signaling Technology, 91144, dilution: 1:1,000), Anti-Collagen III (Abcam, ab7778, dilution: 1:7,000), Anti-α-SMA (Cell Signaling Technology, 19245, dilution: 1:1,000), Anti-p-Smad2 (Cell Signaling Technology, 18338, dilution: 1:1,000), Anti-p-Smad3 (Cell Signaling Technology, 9520, dilution: 1:1,000), Anti-TGF-β2 (Bioss, bs-20412R, dilution: 1:1,000) and Anti-β-actin Antibody (Abcam, ab8227, dilution: 1:1,000), were used.

Techniques: Transfection, Staining, Expressing, Western Blot, Quantitative RT-PCR, Luciferase, Activity Assay, Reporter Assay

Journal: PeerJ

Article Title: CircHIPK3 regulates cardiac fibroblast proliferation, migration and phenotypic switching through the miR-152-3p/TGF-β2 axis under hypoxia

doi: 10.7717/peerj.9796

Figure Lengend Snippet: (A–E) The cell cycle was investigated by a cell cycle assay in cardiac fibroblasts. The results represent the percentage of cells in G1 and S + G2/M phases for the indicated conditions. n = 3. * P < 0.05, # P < 0.05. (F–J) Wound scratch assay for the different groups. The average sizes of the gaps were measured at 48 h. Scale bar = 200 μm. n = 5. * P < 0.05, # P < 0.05. (K and L) The protein expression levels of Col I, Col III, TGF-β2, p-Smad2 and p-Smad3 were measured by western blot analysis. n = 3. * P < 0.05, # P < 0.05. * P < 0.05, vs. hypoxia group, # P < 0.05, vs. si-circHIPK3 group.

Article Snippet: The primary anti-bodies: Anti-Collagen I (Cell Signaling Technology, 91144, dilution: 1:1,000), Anti-Collagen III (Abcam, ab7778, dilution: 1:7,000), Anti-α-SMA (Cell Signaling Technology, 19245, dilution: 1:1,000), Anti-p-Smad2 (Cell Signaling Technology, 18338, dilution: 1:1,000), Anti-p-Smad3 (Cell Signaling Technology, 9520, dilution: 1:1,000), Anti-TGF-β2 (Bioss, bs-20412R, dilution: 1:1,000) and Anti-β-actin Antibody (Abcam, ab8227, dilution: 1:1,000), were used.

Techniques: Cell Cycle Assay, Wound Healing Assay, Expressing, Western Blot

Journal: Toxicology and applied pharmacology

Article Title: Cardiovascular alterations at different stages of hypertension development during ethanol consumption: time-course of vascular and autonomic changes.

doi: 10.1016/j.taap.2014.08.012

Figure Lengend Snippet: Fig. 2. (Left) Changes in mean arterial pressure (ΔMAP) evoked by increasing concentrations of the selective α1-adrenoceptor agonist phenylephrine in animals receiving water (control groups) or increasing concentration of ethanol in drinking water (first week: 5%, second week: 10%, third week: 20%, and fourth week 20% v/v). Circles represent the mean ± SEM. (Right) Maximal effect (Emax) and dose at 50% of the MAP range (ED50) for phenylephrine dose–response curves. The bars represent the mean ± SEM. *P b 0.05 vs respective control group, two- way ANOVA followed by Bonferroni's post hoc test.

Article Snippet: The membranes were blocked by incubation with blocking buffer (10%BSA in TBSt–1.6MNaCl and 0.2MTris, 0.1%Tween 20) for 2 h. After blocking, blots were incubated overnight at 4 °C with primary antibodies to α1a-adrenoceptor (1:1000 dilution; c-19, sc1477, Santa Cruz Biotechnology, Santa Cruz, CA, USA), β2-adrenoceptor (1:500 dilution; M-20, sc-570, Santa Cruz Biotechnology), and β-actin (1:10,000 dilution; 13E5, Rabbit mAB #4970, Cell Signaling Technology).

Techniques: Control, Concentration Assay

Journal: Toxicology and applied pharmacology

Article Title: Cardiovascular alterations at different stages of hypertension development during ethanol consumption: time-course of vascular and autonomic changes.

doi: 10.1016/j.taap.2014.08.012

Figure Lengend Snippet: Fig. 4. Time-course of relative protein levels (protein level/β-actin) of α1a- and β2-adrenoceptor in the mesenteric arterial bed (left) and aorta (right) in animals receiving water (control groups) or increasing concentration of ethanol in drinking water (first week: 5%, second week: 10%, third week: 20%, and fourth week 20% v/v). Circles represent the mean ± SEM. *P b 0.05 vs respective control group, two-way ANOVA followed by Bonferroni's post hoc test.

Article Snippet: The membranes were blocked by incubation with blocking buffer (10%BSA in TBSt–1.6MNaCl and 0.2MTris, 0.1%Tween 20) for 2 h. After blocking, blots were incubated overnight at 4 °C with primary antibodies to α1a-adrenoceptor (1:1000 dilution; c-19, sc1477, Santa Cruz Biotechnology, Santa Cruz, CA, USA), β2-adrenoceptor (1:500 dilution; M-20, sc-570, Santa Cruz Biotechnology), and β-actin (1:10,000 dilution; 13E5, Rabbit mAB #4970, Cell Signaling Technology).

Techniques: Control, Concentration Assay

Journal:

Article Title: Cellular Sources of Transforming Growth Factor-? Isoforms in Early and Chronic Radiation Enteropathy

doi:

Figure Lengend Snippet: Intestinal wall immunoreactivity for TGF-β1 (anti-CC), TGF-β2, and TGF-β3 2 weeks (left) and 26 weeks (right) after 21-Gy single-dose irradiation. Very prominent TGF-β1 immunoreactivity and minimal TGF-β2 and TGF-β3 immunoreactivity is seen at both observation times.

Article Snippet: 31 Antibodies to pan-TGF-β (AB-100-NA), TGF-β2 (AB-12-NA), and TGF-β3 (AB-244-NA) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Irradiation

Journal:

Article Title: Cellular Sources of Transforming Growth Factor-? Isoforms in Early and Chronic Radiation Enteropathy

doi:

Figure Lengend Snippet: In situ hybridization demonstrating TGF-β1 mRNA expression in irradiated intestine. A: TGF-β1 expression in regenerating crypt at the edge of a radiation-induced mucosal ulcer 2 weeks after 21-Gy single-dose irradiation. Magnification, ×200; bar, 100 μm. B: Inflammatory cells and fibroblast-like cells in the base of a radiation-induced mucosal ulcer expressing TGF-β1 2 weeks after 21-Gy single-dose irradiation. Magnification, ×400; bar, 50 μm. C: Intestinal wall beneath a radiation-induced mucosal ulcer (right overview; magnification, ×100; bar, 100 μm). Strong TGF-β1 expression in smooth muscle cells (left upper panel; magnification, ×400) and peritoneal mesothelium (left lower panel; magnification, ×400) is seen 2 weeks after 21-Gy single-dose irradiation. D: TGF-β1 expression in vascular endothelial cells and perivascular cells 26 weeks after 21-Gy single-dose irradiation. Magnification, ×400; bar, 50 μm. E: Fibroblasts in fibrotic area expressing TGF-β1 mRNA 26 weeks after 21-Gy single-dose irradiation. Magnification, ×400; bar, 50 μm.

Article Snippet: 31 Antibodies to pan-TGF-β (AB-100-NA), TGF-β2 (AB-12-NA), and TGF-β3 (AB-244-NA) were purchased from R&D Systems (Minneapolis, MN).

Techniques: In Situ Hybridization, Expressing, Irradiation

Journal:

Article Title: Cellular Sources of Transforming Growth Factor-? Isoforms in Early and Chronic Radiation Enteropathy

doi:

Figure Lengend Snippet: Expression of TGF-β mRNA in Sham-Irradiated and Irradiated Intestine, 2 and 26 Weeks after Irradiation

Article Snippet: 31 Antibodies to pan-TGF-β (AB-100-NA), TGF-β2 (AB-12-NA), and TGF-β3 (AB-244-NA) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Expressing, Irradiation

Journal: Molecular Neurodegeneration

Article Title: Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models

doi: 10.1186/s13024-021-00434-7

Figure Lengend Snippet: Information of the primary antibodies used in this study

Article Snippet: GABAAR-β2 , Abcam , ab156000 , Rabbit , , 1:10000.

Techniques: